The 3â² termini of small RNAs inTrypanosoma brucei
نویسندگان
چکیده
منابع مشابه
The major transcripts of the kinetoplast DNA of Trypanosoma brucei are very small ribosomal RNAs
The nucleotide sequence has been determined of a 2.2 kb segment of kinetoplast DNA, which encodes the major mitochondrial transcripts (12S and 9S) of Trypanosoma brucei. The sequence shows that the 12S RNA is a large subunit rRNA, although sufficiently unusual for resistance to chloramphenicol to be predicted. The 9S RNA has little homology with other rRNAs, but a possible secondary structure i...
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In animals and protozoa, gene-specific double-stranded RNA (dsRNA) triggers degradation of homologous cellular RNAs, a phenomenon known as RNA interference (RNAi). In vitro and in vivo dsRNA is processed by a nuclease to produce 21-25-nt small interfering RNAs (siRNAs) that guide target RNA degradation. Here we show that activation of RNAi in Trypanosoma bruceiby expression or electroporation o...
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Introduction Our understanding of the RNA-mediated gene-regulatory pathway, known as RNA interference (RNAi), has come a long way since its discovery in the early 1990s. At that time, all knowledge of RNAmediated silencing was based on one phenomenon: the loss of a non-lethal phenotype after the introduction of exogenous RNA into the affected organism. Since then, various families of non-coding...
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Pseudogenes have been shown to acquire unique regulatory roles from more and more organisms. We report the observation of a cluster of siRNAs derived from pseudogenes of African Trypanosoma brucei using high through-put analysis. We show that these pseudogene-derived siRNAs suppress gene expression through RNA interference. The discovery that siRNAs may originate from pseudogenes and regulate g...
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For several decades, only a limited number of noncoding RNAs, such as ribosomal and transfer RNA, have been studied in any depth. In recent years, additional species of noncoding RNAs have increasingly been discovered. Of these, small RNA species attract particular interest because of their essential roles in processes such as RNA silencing and modifications. Detailed analyses revealed several ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2004
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2004.tb09629.x